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The key project

 

Project name: Discovering novel Bt strains and Bt toxic proteins for better bio-pesticides and biotech crops


Bacillus thuringiensis (Bt) is the most successful biological pesticide bacteria up to now. Its genes, which coding the insecticidal toxin had been successfully applied to plant transgenosis, became one of the most effective foreign "molecular properties" for plants. The research project was started in 2005, separated the Bt from soil samples which was collected in the tropical rainforests, then identified and separated the toxin genes. Discovering novel Bt strains and Bt toxic proteins for better bio-pesticides and biotech crops.

Bt resources in HITAR

32,250 Bacillus isolated strains
2,820 Bt isolated strains
450 Bt strains have been studied in detail
60 Bt strains have been genome-wide sequenced
10 Bt strains was recommended as alternative strains for the existing model strains

Mainly project in research

Separate, identify, genome sequencing and bioassay of Bt strain

Cloning and expression of insecticidal toxin gene in Bt strains

Gene cloning and application of “mosquito killing” Bt strains
Gene cloning and application of “nematode killing” Bt strains
Bt toxin protein and gene used in biological pesticide and transgenic plant

Bt strains used in biological pesticides and toxic protein genes applied to Bt crops

20 Bt strains can be used to kill lepidoptera insects, target insects: Prodenia litura, Corn borer, Chilo suppressalis Walker and the cotton bollworm
10 Bt strains can be used to kill coleoptera insects, target insects: Leptinotarsa decemlineata, Brontispa longissima Gestro and Sugarcane borers
30 Bt strains can be used to kill 30 diptera insects, target insects: Culex and Anopheles
7 Bt strains can be used to kill the insect species, target type: Necator americanus, root eelworm and Heterodera glycines Ichinohe
Cry1Ac is used to cultivating Bt cotton, resistance to bollworms
Cry1Ab is used to cultivating Bt rice, resistance to rice Chilo suppressalis Walker

Awarded project

From 17 Sep. 2013 to 19 Sep. 2013, the 13th Agricultural Biotechnology International Conference (ABIC 2013) was convened in Calgary alberta, Canada. The only award of this conference was awarded to the scientific research project “Discovering Novel B.t. Toxins for Better Bio-pesticides and Biotech Crops”, which belongs to Dr. Xuanjun Fang, HITAR.

424 delegates from 23 countries attended this conference, discussed the agricultural biotechnology in the role of agriculture, energy, prevention and healthcare (human and animal), and investment. With strict selection, the jury of the conference selected out the only winner from all of the excellent research projects belongs to health and agricultural (food), environment/global management or productivity/quality.